Journal:

Article Title: In Vitro Activities and Postantifungal Effects of the Potent Dolastatin 10 Derivative Auristatin PHE

doi: 10.1128/AAC.45.12.3580-3584.2001

Figure Lengend Snippet: Broth microdilution MICs and MFCs of auristatin PHE and dolastatin 10 for reference strains and clinical isolates

Article Snippet: With a large number of species closely related to C. neoformans , we have now confirmed this result for dolastatin 10 (Table ). table ft1 table-wrap mode="anchored" t5 caption a7 Organism (no. of strains) MIC (MFC) (μg/ml) Auristatin PHE Dolastatin 10 Blastoschizomyces capitatus ATCC 10663 >64 >64 Bulleromyces albus ATCC 18568 >64 >64 Candida albicans ATCC 32354 a >64 >64 Candida albicans ATCC 64124 b >64 >64 Candida albicans (2) >64 >64 Candida glabrata (2) >64 >64 Candida guillermondii (1) >64 >64 Candida krusei (2) >64 >64 Candida lusitaneae ATCC 42720 c >64 >64 Candida parapsilosis (2) >64 >64 Candida rugosa (1) >64 >64 Candida tropicalis ATCC 66029 >64 >64 Candida tropicalis (2) >64 >64 Candida utilis ATCC 22020 >64 >64 Candida viswanathii ATCC 22977 >64 >64 Cryptococcus albidus ATCC 10666 >64 >64 Cryptococcus albidus ATCC 34140 >64 >64 Cryptococcus albidus ATCC 66030 >64 >64 Cryptococcus ater ATCC 14247 >64 >64 Cryptococcus humicolus ATCC 9949 >64 >64 Cryptococcus laurentii ATCC 18803 >64 >64 Cryptococcus laurentii ATCC 34142 >64 >64 Cryptococcus laurentii ATCC 66036 >64 >64 Cryptococcus neoformans ATCC 90112 0.5 (0.5) 4 (4) Cryptococcus neoformans (10) 0.5–1 (0.5–1) 2–8 (2–8) Cryptococcus neoformans 94-2406 a 0.0156 (0.0156) 0.25 (0.5) Cryptococcus neoformans 94-2483 a 0.0625 (0.0625) 1 (1) Cryptococcus neoformans 95-2792 a 0.5 (0.5) 16 (64) Cryptococcus neoformans 96-2011 a 0.5 (2) 32 (32) Cryptococcus unguttulatus ATCC 34143 >64 >64 Cryptococcus unguttulatus ATCC 66033 >64 >64 Filobasidium uniguttulatum ATCC 24227 >64 >64 Geotrichum candidum ATCC 34614 >64 >64 Kluyveromyces apiculate ATCC 9774 >64 >64 Kluyveromyces marxianus ATCC 36534 >64 >64 Pichia anomala ATCC 2349 >64 >64 Malassezia furfur ATCC 44344 >64 >64 Rhodotorula glutinis ATCC 32765 >64 >64 Rhodotorula mucilaginosa ATCC 9449 >64 >64 Saccharomyces cerevisiae (3) >64 >64 Trichosporon asahii ATCC 20039 >64 >64 Trichosporon asahii T6991-3 8 (16) >64 Trichosporon cutaneum ATCC 28592 16 (>64) >64 Trichosporon inkin ATCC 18020 16 (>64) >64 Trichosporon mucoides ATCC 90046 64 (64) >64 Trichosporon ovoides ATCC 90040 4 (4) >64 Aspergillus flavus ASU-CC d >64 >64 Aspergillus fumigatus ATCC 96918 >64 >64 Aspergillus nidulans FGSC-4 e >64 >64 Aspergillus niger ASU-CC >64 >64 Paecilomyces lilacinus ASU-CC >64 >64 Rhizopus nigricans ASU-CC >64 >64 Rhizopus oligosporus ATCC 22959 >64 >64 Open in a separate window a Fluconazole-resistant strain. b Ketoconazole-resistant strain. c Amphotericin B-resistant strain. d ASU-CC, Arizona State University Culture Collection. e FGSC, Fungal Glasgow Strain Collection.

Techniques:

Journal:

Article Title: Genetic Diversity and Biochemical Characteristics of Trichosporon asahii Isolated from Clinical Specimens, Houses of Patients with Summer-Type-Hypersensitivity Pneumonitis, and Environmental Materials

doi: 10.1128/JCM.39.7.2405-2411.2001

Figure Lengend Snippet: Specificity of primers M13-7F and M13-7R for Trichosporon species and related species

Article Snippet: Table shows the strains used for specificity of PCR. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Strain a PCR product b Trichosporon coremiiforme CBS 2482 — Trichosporon faecale CBS 4828 — Trichosporon asteroides CBS 2481 — Trichosporon aquatile CBS 5973 — Trichosporon brassicae CBS 6382 — Trichosporon cutaneum CBS 2466 — Trichosporon dulcitum CBS 8257 — Trichosporon inkin CBS 5585 — Trichosporon gracile CBS 8189 — Trichosporon montevideense CBS 6721 — Trichosporon mucoides CBS 7625 — Trichosporon ovoides CBS 5585 — Trichosporon pullulans CBS 2532 — Candida albicans CBS 562 — Candida glabrata IFO 0622 — Candida guilliermondii CBS 566 — Candida kefyr JCM 9559 — Candida lusitaniae CBS 4413 — Candida parapsilosis ATCC 22019 — Candida tropicalis ATCC 7349 — Cryptococcus neoformans var. neoformans CBS 132 — Cryptococcus neoformans var. gattii NIH 191 — Cryptococcus albidus CBS 142 — Malassezia furfur CBS 1878 — Rhodotorula mucilaginosa CBS 17 — Open in a separate window a Abbreviations: CBS, Centraal Bureau voor Schimmelcultures, Baarn, The Netherlands; IFO, Institute for Fermentation, Osaka, Japan; JCM, Japan Collection of Microorganisms, Saitama, Japan; NIH, National Institutes for Health, Bethesda, Md. b —, no product obtained.

Techniques:

Journal:

Article Title: Genetic Diversity and Biochemical Characteristics of Trichosporon asahii Isolated from Clinical Specimens, Houses of Patients with Summer-Type-Hypersensitivity Pneumonitis, and Environmental Materials

doi: 10.1128/JCM.39.7.2405-2411.2001

Figure Lengend Snippet: Specificity of primers M13-7F and M13-7R for Trichosporon species and related species

Article Snippet: Table shows the strains used for specificity of PCR. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Strain a PCR product b Trichosporon coremiiforme CBS 2482 — Trichosporon faecale CBS 4828 — Trichosporon asteroides CBS 2481 — Trichosporon aquatile CBS 5973 — Trichosporon brassicae CBS 6382 — Trichosporon cutaneum CBS 2466 — Trichosporon dulcitum CBS 8257 — Trichosporon inkin CBS 5585 — Trichosporon gracile CBS 8189 — Trichosporon montevideense CBS 6721 — Trichosporon mucoides CBS 7625 — Trichosporon ovoides CBS 5585 — Trichosporon pullulans CBS 2532 — Candida albicans CBS 562 — Candida glabrata IFO 0622 — Candida guilliermondii CBS 566 — Candida kefyr JCM 9559 — Candida lusitaniae CBS 4413 — Candida parapsilosis ATCC 22019 — Candida tropicalis ATCC 7349 — Cryptococcus neoformans var. neoformans CBS 132 — Cryptococcus neoformans var. gattii NIH 191 — Cryptococcus albidus CBS 142 — Malassezia furfur CBS 1878 — Rhodotorula mucilaginosa CBS 17 — Open in a separate window a Abbreviations: CBS, Centraal Bureau voor Schimmelcultures, Baarn, The Netherlands; IFO, Institute for Fermentation, Osaka, Japan; JCM, Japan Collection of Microorganisms, Saitama, Japan; NIH, National Institutes for Health, Bethesda, Md. b —, no product obtained.

Techniques:

Journal:

Article Title: Genetic Diversity and Biochemical Characteristics of Trichosporon asahii Isolated from Clinical Specimens, Houses of Patients with Summer-Type-Hypersensitivity Pneumonitis, and Environmental Materials

doi: 10.1128/JCM.39.7.2405-2411.2001

Figure Lengend Snippet: Specificity of primers M13-7F and M13-7R for Trichosporon species and related species

Article Snippet: Table shows the strains used for specificity of PCR. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Species Strain a PCR product b Trichosporon coremiiforme CBS 2482 — Trichosporon faecale CBS 4828 — Trichosporon asteroides CBS 2481 — Trichosporon aquatile CBS 5973 — Trichosporon brassicae CBS 6382 — Trichosporon cutaneum CBS 2466 — Trichosporon dulcitum CBS 8257 — Trichosporon inkin CBS 5585 — Trichosporon gracile CBS 8189 — Trichosporon montevideense CBS 6721 — Trichosporon mucoides CBS 7625 — Trichosporon ovoides CBS 5585 — Trichosporon pullulans CBS 2532 — Candida albicans CBS 562 — Candida glabrata IFO 0622 — Candida guilliermondii CBS 566 — Candida kefyr JCM 9559 — Candida lusitaniae CBS 4413 — Candida parapsilosis ATCC 22019 — Candida tropicalis ATCC 7349 — Cryptococcus neoformans var. neoformans CBS 132 — Cryptococcus neoformans var. gattii NIH 191 — Cryptococcus albidus CBS 142 — Malassezia furfur CBS 1878 — Rhodotorula mucilaginosa CBS 17 — Open in a separate window a Abbreviations: CBS, Centraal Bureau voor Schimmelcultures, Baarn, The Netherlands; IFO, Institute for Fermentation, Osaka, Japan; JCM, Japan Collection of Microorganisms, Saitama, Japan; NIH, National Institutes for Health, Bethesda, Md. b —, no product obtained.

Techniques:

Journal:

Article Title: Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions

doi:

Figure Lengend Snippet: Specificity of the primers for T. asahii and related species

Article Snippet: The reaction mixtures were amplified in a Perkin-Elmer 9700 thermal cycler, using the following program: 94°C for 3 min, followed by 30 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 72°C for 40 min, with a final extension period at 72°C for 10 min. After thermal cycling, 3 μl of the amplified product was run on a 1.5% (wt/vol) agarose gel, stained with ethidium bromide, and visualized under a UV light. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain Source a PCR product b T. asahii var. asahii M 9306 c CBS 2479 + M 9402 Blood + M 9403 Blood + M 9415 Lung + M 9416 Lung + M 9417 Lung + M 9411 Sputum + M 9410 Feces + M 9405 Urine + M 9406 Urine + T. asahii var. coremiformis M 9309 c CBS 2482 + M 9404 Urine + T. asahii var. faecalis M 9312 c CBS 4828 + M 9409 Urine + T. asteroides M 9308 c CBS 2481 − M 9329 CBS 7623 − M 9330 CBS 7624 − T. aquatile M 9317 c CBS 5973 − M 9321 CBS 5988 − T. brassicae M 9322 c CBS 6382 − T. cutaneum M 9304 c CBS 2466 − M 9307 CBS 2480 − M 9423 Skin − M 9425 Skin − T. domesticum M 9401 c House − M 9412 Nail − T. dulcitum M 9337 c CBS 8257 − T. inkin M 9316 c CBS 5585 − M 9333 CBS 7926 − T. gracile M 9334 c CBS 8189 − T. jirovecii M 9326 c CBS 6864 − T. loubieri var. loubieri M 9327 c CBS 7065 − T. loubieri var. laibachii M 9319 c CBS 5790 − T. montevideense M 9323 c CBS 6721 − T. mucoides M 9331 c CBS 7625 − M 9332 CBS 7626 − M 9422 Skin − M 9424 Skin − T. moniliiforme M 9305 c CBS 2467 − T. ovoides M 9328 c CBS 5585 − M 9315 CBS 5580 − M 9407 Urine − T. pullulans M 9339 c CBS 2532 − M 9440 CBS 2533 − M 9441 CBS 2538 − T. sporotrichoides M 9336 c CBS 8245 − Candida albicans M 1001 c CBS 562 − M 1447 NIHA207 − M 1445 NIHB792 − Candida glabrata M 4002 c IFO 0622 − Candida guilliermondii M 1002 c CBS 566 − M 2054 Skin − M 1023 ATCC 9058 − Candida kefyr M 1018 c JCM 9559 − Candida lusitaniae M 1010 c CBS 4413 − Candida parapsilosis M 1015 c ATCC 22019 − Candida tropicalis M 1017 c ATCC 7349 − Cryptococcus neoformans var. neoformans M 9010 c CBS 132 − M 9026 NIHB3501 − M 9116 Cerebrospinal fluid − M 9117 Skin − M 9125 VUT 77063 − M 9216 Cerebrospinal fluid − Cryptococcus neoformans var. gattii M 9028 NIH191 − M 9228 CDCB3174 − M 9229 CDCB3175 − M 9230 CDCB3183 − M 9246 CDCB3184 − M 9247 CDCB3185 − Cryptococcus albidus M 9001 c CBS 142 − Debaryomyces hansenii var. hansenii M 5012 c JCM 1990 − M 5111 CBS 1793 − M 5033 JCM 1521 − Malassezia furfur M 9701 c CBS 1878 − M 9702 CBS 6093 − M 9703 CBS 7019 − Malassezia pachydermatis M 9704 c CBS 1079 − Rhodotorula mucilaginosa M 9501 c CBS 17 − Open in a separate window a Abbreviations: ATCC, American Type Culture Collection, Rockville, Md.

Techniques:

Journal:

Article Title: Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions

doi:

Figure Lengend Snippet: Specificity of the primers for T. asahii and related species

Article Snippet: The reaction mixtures were amplified in a Perkin-Elmer 9700 thermal cycler, using the following program: 94°C for 3 min, followed by 30 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 72°C for 40 min, with a final extension period at 72°C for 10 min. After thermal cycling, 3 μl of the amplified product was run on a 1.5% (wt/vol) agarose gel, stained with ethidium bromide, and visualized under a UV light. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain Source a PCR product b T. asahii var. asahii M 9306 c CBS 2479 + M 9402 Blood + M 9403 Blood + M 9415 Lung + M 9416 Lung + M 9417 Lung + M 9411 Sputum + M 9410 Feces + M 9405 Urine + M 9406 Urine + T. asahii var. coremiformis M 9309 c CBS 2482 + M 9404 Urine + T. asahii var. faecalis M 9312 c CBS 4828 + M 9409 Urine + T. asteroides M 9308 c CBS 2481 − M 9329 CBS 7623 − M 9330 CBS 7624 − T. aquatile M 9317 c CBS 5973 − M 9321 CBS 5988 − T. brassicae M 9322 c CBS 6382 − T. cutaneum M 9304 c CBS 2466 − M 9307 CBS 2480 − M 9423 Skin − M 9425 Skin − T. domesticum M 9401 c House − M 9412 Nail − T. dulcitum M 9337 c CBS 8257 − T. inkin M 9316 c CBS 5585 − M 9333 CBS 7926 − T. gracile M 9334 c CBS 8189 − T. jirovecii M 9326 c CBS 6864 − T. loubieri var. loubieri M 9327 c CBS 7065 − T. loubieri var. laibachii M 9319 c CBS 5790 − T. montevideense M 9323 c CBS 6721 − T. mucoides M 9331 c CBS 7625 − M 9332 CBS 7626 − M 9422 Skin − M 9424 Skin − T. moniliiforme M 9305 c CBS 2467 − T. ovoides M 9328 c CBS 5585 − M 9315 CBS 5580 − M 9407 Urine − T. pullulans M 9339 c CBS 2532 − M 9440 CBS 2533 − M 9441 CBS 2538 − T. sporotrichoides M 9336 c CBS 8245 − Candida albicans M 1001 c CBS 562 − M 1447 NIHA207 − M 1445 NIHB792 − Candida glabrata M 4002 c IFO 0622 − Candida guilliermondii M 1002 c CBS 566 − M 2054 Skin − M 1023 ATCC 9058 − Candida kefyr M 1018 c JCM 9559 − Candida lusitaniae M 1010 c CBS 4413 − Candida parapsilosis M 1015 c ATCC 22019 − Candida tropicalis M 1017 c ATCC 7349 − Cryptococcus neoformans var. neoformans M 9010 c CBS 132 − M 9026 NIHB3501 − M 9116 Cerebrospinal fluid − M 9117 Skin − M 9125 VUT 77063 − M 9216 Cerebrospinal fluid − Cryptococcus neoformans var. gattii M 9028 NIH191 − M 9228 CDCB3174 − M 9229 CDCB3175 − M 9230 CDCB3183 − M 9246 CDCB3184 − M 9247 CDCB3185 − Cryptococcus albidus M 9001 c CBS 142 − Debaryomyces hansenii var. hansenii M 5012 c JCM 1990 − M 5111 CBS 1793 − M 5033 JCM 1521 − Malassezia furfur M 9701 c CBS 1878 − M 9702 CBS 6093 − M 9703 CBS 7019 − Malassezia pachydermatis M 9704 c CBS 1079 − Rhodotorula mucilaginosa M 9501 c CBS 17 − Open in a separate window a Abbreviations: ATCC, American Type Culture Collection, Rockville, Md.

Techniques:

Journal:

Article Title: Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions

doi:

Figure Lengend Snippet: Agarose gel electrophoresis of PCR products of T. asahii and related species. Lane 1, molecular size marker (X174 DNA, HaeIII digest; Takara, Shiga, Japan); lane 2, T. asahii var. asahii M 9306; lane 3, T. asahii var. coremiformis M 9309; lane 4, T. asahii var. faecalis M 9312; lane 5, clinical isolate T. asahii var. asahii M 9402; lane 6, clinical isolate T. asahii var. asahii M 9415; lane 7, clinical isolate T. asahii var. asahii M 9411; lane 8, clinical isolate T. asahii var. asahii M 9405; lane 9, T. asteroides M 9308; lane 10, T. ovoides M 9328; lane 11, T. mucoides M 9331; lane 12, T. cutaneum M 9304; lane 13, Candida albicans M 1001; lane 14, Candida parapsilosis M 1015; lane 15, Cryptococcus neoformans var. neoformans M 9010; lane 16, Cryptococcus neoformans var. gattii M 9028; lane 17, M. furfur M 9701.

Article Snippet: The reaction mixtures were amplified in a Perkin-Elmer 9700 thermal cycler, using the following program: 94°C for 3 min, followed by 30 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 72°C for 40 min, with a final extension period at 72°C for 10 min. After thermal cycling, 3 μl of the amplified product was run on a 1.5% (wt/vol) agarose gel, stained with ethidium bromide, and visualized under a UV light. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain Source a PCR product b T. asahii var. asahii M 9306 c CBS 2479 + M 9402 Blood + M 9403 Blood + M 9415 Lung + M 9416 Lung + M 9417 Lung + M 9411 Sputum + M 9410 Feces + M 9405 Urine + M 9406 Urine + T. asahii var. coremiformis M 9309 c CBS 2482 + M 9404 Urine + T. asahii var. faecalis M 9312 c CBS 4828 + M 9409 Urine + T. asteroides M 9308 c CBS 2481 − M 9329 CBS 7623 − M 9330 CBS 7624 − T. aquatile M 9317 c CBS 5973 − M 9321 CBS 5988 − T. brassicae M 9322 c CBS 6382 − T. cutaneum M 9304 c CBS 2466 − M 9307 CBS 2480 − M 9423 Skin − M 9425 Skin − T. domesticum M 9401 c House − M 9412 Nail − T. dulcitum M 9337 c CBS 8257 − T. inkin M 9316 c CBS 5585 − M 9333 CBS 7926 − T. gracile M 9334 c CBS 8189 − T. jirovecii M 9326 c CBS 6864 − T. loubieri var. loubieri M 9327 c CBS 7065 − T. loubieri var. laibachii M 9319 c CBS 5790 − T. montevideense M 9323 c CBS 6721 − T. mucoides M 9331 c CBS 7625 − M 9332 CBS 7626 − M 9422 Skin − M 9424 Skin − T. moniliiforme M 9305 c CBS 2467 − T. ovoides M 9328 c CBS 5585 − M 9315 CBS 5580 − M 9407 Urine − T. pullulans M 9339 c CBS 2532 − M 9440 CBS 2533 − M 9441 CBS 2538 − T. sporotrichoides M 9336 c CBS 8245 − Candida albicans M 1001 c CBS 562 − M 1447 NIHA207 − M 1445 NIHB792 − Candida glabrata M 4002 c IFO 0622 − Candida guilliermondii M 1002 c CBS 566 − M 2054 Skin − M 1023 ATCC 9058 − Candida kefyr M 1018 c JCM 9559 − Candida lusitaniae M 1010 c CBS 4413 − Candida parapsilosis M 1015 c ATCC 22019 − Candida tropicalis M 1017 c ATCC 7349 − Cryptococcus neoformans var. neoformans M 9010 c CBS 132 − M 9026 NIHB3501 − M 9116 Cerebrospinal fluid − M 9117 Skin − M 9125 VUT 77063 − M 9216 Cerebrospinal fluid − Cryptococcus neoformans var. gattii M 9028 NIH191 − M 9228 CDCB3174 − M 9229 CDCB3175 − M 9230 CDCB3183 − M 9246 CDCB3184 − M 9247 CDCB3185 − Cryptococcus albidus M 9001 c CBS 142 − Debaryomyces hansenii var. hansenii M 5012 c JCM 1990 − M 5111 CBS 1793 − M 5033 JCM 1521 − Malassezia furfur M 9701 c CBS 1878 − M 9702 CBS 6093 − M 9703 CBS 7019 − Malassezia pachydermatis M 9704 c CBS 1079 − Rhodotorula mucilaginosa M 9501 c CBS 17 − Open in a separate window a Abbreviations: ATCC, American Type Culture Collection, Rockville, Md.

Techniques: Agarose Gel Electrophoresis, Marker

Journal:

Article Title: Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions

doi:

Figure Lengend Snippet: Specificity of the primers for T. asahii and related species

Article Snippet: The reaction mixtures were amplified in a Perkin-Elmer 9700 thermal cycler, using the following program: 94°C for 3 min, followed by 30 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 72°C for 40 min, with a final extension period at 72°C for 10 min. After thermal cycling, 3 μl of the amplified product was run on a 1.5% (wt/vol) agarose gel, stained with ethidium bromide, and visualized under a UV light. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain Source a PCR product b T. asahii var. asahii M 9306 c CBS 2479 + M 9402 Blood + M 9403 Blood + M 9415 Lung + M 9416 Lung + M 9417 Lung + M 9411 Sputum + M 9410 Feces + M 9405 Urine + M 9406 Urine + T. asahii var. coremiformis M 9309 c CBS 2482 + M 9404 Urine + T. asahii var. faecalis M 9312 c CBS 4828 + M 9409 Urine + T. asteroides M 9308 c CBS 2481 − M 9329 CBS 7623 − M 9330 CBS 7624 − T. aquatile M 9317 c CBS 5973 − M 9321 CBS 5988 − T. brassicae M 9322 c CBS 6382 − T. cutaneum M 9304 c CBS 2466 − M 9307 CBS 2480 − M 9423 Skin − M 9425 Skin − T. domesticum M 9401 c House − M 9412 Nail − T. dulcitum M 9337 c CBS 8257 − T. inkin M 9316 c CBS 5585 − M 9333 CBS 7926 − T. gracile M 9334 c CBS 8189 − T. jirovecii M 9326 c CBS 6864 − T. loubieri var. loubieri M 9327 c CBS 7065 − T. loubieri var. laibachii M 9319 c CBS 5790 − T. montevideense M 9323 c CBS 6721 − T. mucoides M 9331 c CBS 7625 − M 9332 CBS 7626 − M 9422 Skin − M 9424 Skin − T. moniliiforme M 9305 c CBS 2467 − T. ovoides M 9328 c CBS 5585 − M 9315 CBS 5580 − M 9407 Urine − T. pullulans M 9339 c CBS 2532 − M 9440 CBS 2533 − M 9441 CBS 2538 − T. sporotrichoides M 9336 c CBS 8245 − Candida albicans M 1001 c CBS 562 − M 1447 NIHA207 − M 1445 NIHB792 − Candida glabrata M 4002 c IFO 0622 − Candida guilliermondii M 1002 c CBS 566 − M 2054 Skin − M 1023 ATCC 9058 − Candida kefyr M 1018 c JCM 9559 − Candida lusitaniae M 1010 c CBS 4413 − Candida parapsilosis M 1015 c ATCC 22019 − Candida tropicalis M 1017 c ATCC 7349 − Cryptococcus neoformans var. neoformans M 9010 c CBS 132 − M 9026 NIHB3501 − M 9116 Cerebrospinal fluid − M 9117 Skin − M 9125 VUT 77063 − M 9216 Cerebrospinal fluid − Cryptococcus neoformans var. gattii M 9028 NIH191 − M 9228 CDCB3174 − M 9229 CDCB3175 − M 9230 CDCB3183 − M 9246 CDCB3184 − M 9247 CDCB3185 − Cryptococcus albidus M 9001 c CBS 142 − Debaryomyces hansenii var. hansenii M 5012 c JCM 1990 − M 5111 CBS 1793 − M 5033 JCM 1521 − Malassezia furfur M 9701 c CBS 1878 − M 9702 CBS 6093 − M 9703 CBS 7019 − Malassezia pachydermatis M 9704 c CBS 1079 − Rhodotorula mucilaginosa M 9501 c CBS 17 − Open in a separate window a Abbreviations: ATCC, American Type Culture Collection, Rockville, Md.

Techniques:

Journal:

Article Title: Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions

doi:

Figure Lengend Snippet: Specificity of the primers for T. asahii and related species

Article Snippet: The reaction mixtures were amplified in a Perkin-Elmer 9700 thermal cycler, using the following program: 94°C for 3 min, followed by 30 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 72°C for 40 min, with a final extension period at 72°C for 10 min. After thermal cycling, 3 μl of the amplified product was run on a 1.5% (wt/vol) agarose gel, stained with ethidium bromide, and visualized under a UV light. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain Source a PCR product b T. asahii var. asahii M 9306 c CBS 2479 + M 9402 Blood + M 9403 Blood + M 9415 Lung + M 9416 Lung + M 9417 Lung + M 9411 Sputum + M 9410 Feces + M 9405 Urine + M 9406 Urine + T. asahii var. coremiformis M 9309 c CBS 2482 + M 9404 Urine + T. asahii var. faecalis M 9312 c CBS 4828 + M 9409 Urine + T. asteroides M 9308 c CBS 2481 − M 9329 CBS 7623 − M 9330 CBS 7624 − T. aquatile M 9317 c CBS 5973 − M 9321 CBS 5988 − T. brassicae M 9322 c CBS 6382 − T. cutaneum M 9304 c CBS 2466 − M 9307 CBS 2480 − M 9423 Skin − M 9425 Skin − T. domesticum M 9401 c House − M 9412 Nail − T. dulcitum M 9337 c CBS 8257 − T. inkin M 9316 c CBS 5585 − M 9333 CBS 7926 − T. gracile M 9334 c CBS 8189 − T. jirovecii M 9326 c CBS 6864 − T. loubieri var. loubieri M 9327 c CBS 7065 − T. loubieri var. laibachii M 9319 c CBS 5790 − T. montevideense M 9323 c CBS 6721 − T. mucoides M 9331 c CBS 7625 − M 9332 CBS 7626 − M 9422 Skin − M 9424 Skin − T. moniliiforme M 9305 c CBS 2467 − T. ovoides M 9328 c CBS 5585 − M 9315 CBS 5580 − M 9407 Urine − T. pullulans M 9339 c CBS 2532 − M 9440 CBS 2533 − M 9441 CBS 2538 − T. sporotrichoides M 9336 c CBS 8245 − Candida albicans M 1001 c CBS 562 − M 1447 NIHA207 − M 1445 NIHB792 − Candida glabrata M 4002 c IFO 0622 − Candida guilliermondii M 1002 c CBS 566 − M 2054 Skin − M 1023 ATCC 9058 − Candida kefyr M 1018 c JCM 9559 − Candida lusitaniae M 1010 c CBS 4413 − Candida parapsilosis M 1015 c ATCC 22019 − Candida tropicalis M 1017 c ATCC 7349 − Cryptococcus neoformans var. neoformans M 9010 c CBS 132 − M 9026 NIHB3501 − M 9116 Cerebrospinal fluid − M 9117 Skin − M 9125 VUT 77063 − M 9216 Cerebrospinal fluid − Cryptococcus neoformans var. gattii M 9028 NIH191 − M 9228 CDCB3174 − M 9229 CDCB3175 − M 9230 CDCB3183 − M 9246 CDCB3184 − M 9247 CDCB3185 − Cryptococcus albidus M 9001 c CBS 142 − Debaryomyces hansenii var. hansenii M 5012 c JCM 1990 − M 5111 CBS 1793 − M 5033 JCM 1521 − Malassezia furfur M 9701 c CBS 1878 − M 9702 CBS 6093 − M 9703 CBS 7019 − Malassezia pachydermatis M 9704 c CBS 1079 − Rhodotorula mucilaginosa M 9501 c CBS 17 − Open in a separate window a Abbreviations: ATCC, American Type Culture Collection, Rockville, Md.

Techniques: